![]() ![]() Plant Dis 87:1411–1415Ĭhen J, Chen J, Adams MJ (2001) Molecular characterisation of a complex mixture of viruses in garlic with mosaic symptoms in China. Iran J Basic Med Sci 16:1031–1048Ĭonci VC, Canavelli A, Lunello P, Di Rienzo J, Nome SF, Zumelzu G, Italia R (2003) Yield losses associated with virus-infected garlic plants during five successive years. Mikaili P, Maadirad S, Moloudizargari M, Aghajanshakeri S, Sarahroodi S (2013) Therapeutic uses and pharmacological properties of garlic, shallot, and their biologically active compounds. This is the first report of GYCV in China. ![]() Differences between the phylogenies inferred for the replicase and coat protein indicate that the new virus does not belong to any established genus of the family Betaflexiviridae. Phylogenetic analysis suggested that it represents an independent evolutionary lineage in the subfamily, clustering with the currently unclassified garlic yellow mosaic associated virus (GYMaV) and peony betaflexivirus 1 (PeV1). The virus has been tentatively named "garlic yellow curl virus" (GYCV). These open reading frames encode the viral replicase, triple gene block, and coat protein, and the genome organization is typical of members of the subfamily Quinvirinae. The complete RNA genome (GenBank accession number OP021693) is 8191 nucleotides in length, excluding the 3’ poly(A) tail, and contains five open reading frames (ORFs). The complete genome of a new virus belonging to the family Betaflexiviridae was identified in garlic and sequenced by next-generation sequencing and reverse transcription PCR. ![]()
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